Exploring the use of patients own fat to stimulate tissue regeneration in the pelvic floor
BAUS ePoster online library. Mironska E. Jun 26, 2018; 211325
Disclosure(s): I have received a 2 year research grant from The Urology Foundation for my work.
Emma Mironska
Emma Mironska
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Abstract
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Introduction: Our aim is to develop a new biodegradable material consisting of polylactic acid (PLA) scaffold augmented with patients' adipose derived mesenchymal stem cells (ADMSCs). Synthetic, transvaginal mesh made of polypropylene (PPL) has been the mainstay of pelvic organ prolapse surgery but is now recognised to lead to severe complications in a significant proportion of woman. Therefore, there is an urgent need for new materials for use within the pelvic floor. An ideal material would be strong enough to provide support to prolapsed pelvic organs but not so stiff as to induce erosion through native tissues (as currently occurs with PPL). For tissue integration and remodelling of patient's tissues it should also promote angiogenesis, reduce inflammation and increase collagen production.

Materials and Methods: We used electrospun PLA which provides good mechanical support and degrades slowly without provoking a strong inflammatory response. ADMSCs have been shown to promote angiogenesis and reduce inflammation while promoting connective tissue regeneration. Using cells harvested from donated human fat, we have isolated a cell rich stromal vascular fraction (SVF) containing both ADMSCs and other progenitor cells. The SVF can be harvested both enzymatically and mechanically.

Results: We have developed a biodegradable polylactic acid (PLA) electrospun scaffold containing pockets into which ADMSCs can be placed We report that ADMSCs attach and proliferate well within PLA pockets, remaining viable and metabolically active.

Conclusion: We report the first steps to achieving a material which can be combined with patients' cells for supporting regeneration of the pelvic floor in one single operation.
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