9p chromosomal deletion on array-based copy number analysis predicts recurrence in non-metastatic clear cell renal cell carcinoma (ccRCC) following surgical resection
BAUS ePoster online library. Quddus B.
Jun 27, 2018; 211376
Mr. Bilal Quddus
Mr. Bilal Quddus
Login now to access Regular content available to all registered users.

You may also access this content "anytime, anywhere" with the Free MULTILEARNING App for iOS and Android
Abstract
Rate & Comment (0)
Background: Copy number variations (CNVs) affect biological and clinical behavior of cancers. The aim of this study was to assess CNVs in tumour tissue of a historic group of patients with clear cell RCC.

Methods: 38 consecutive ccRCC cases with median follow up of 81.5 months were identified from Tayside Tissue Bank archive. Histology was confirmed by specialist uro-pathologist. DNA extracted from Fresh frozen samples in 33 cases was analysed with CytoChipOligo™ BlueGenome (Illumina®). Affymetrix OncoScan® FFPE assay was employed to analyse highly degraded DNA extracted from 5 FFPE samples. Nexus software (Biodiscovery®) version 7.5 was used to analyse array based data. Cox-proportional hazard survival analysis for recurrence-free survival (RFS) was performed.

Results: Mean age of cohort was 65 years. Loss of 3p was detected in 95% of the tumours. Other common chromosomal CNVs detected were deletions of 4q (34%), 9p (34%), 14q (34%) and 18q (34%). Common gains included 5q (50%) and 7q (23%). Only 9p deletion was associated with high grade tumours. Ten out of 32 cases with localised ccRCC at diagnosis developed recurrence, with a median follow up of 86.5 months. Out of all clinico-pathological and CNVs, somatic deletion of chromosome 9p was the only significant predictor of recurrence (p=0.002; Hazard ratio: 8.8).

Conclusion: The study confirms that 9p deletion is one of the most common CNVs in ccRCC. Moreover, this is the first array based analysis study, with follow up exceeding 7 years, showing that 9p deletion is associated with higher of recurrence in non-metastatic ccRCC.
    This eLearning portal is powered by:
    This eLearning portal is powered by MULTIEPORTAL
Anonymous User Privacy Preferences

Strictly Necessary Cookies (Always Active)

MULTILEARNING platforms and tools hereinafter referred as “MLG SOFTWARE” are provided to you as pure educational platforms/services requiring cookies to operate. In the case of the MLG SOFTWARE, cookies are essential for the Platform to function properly for the provision of education. If these cookies are disabled, a large subset of the functionality provided by the Platform will either be unavailable or cease to work as expected. The MLG SOFTWARE do not capture non-essential activities such as menu items and listings you click on or pages viewed.


Performance Cookies

Performance cookies are used to analyse how visitors use a website in order to provide a better user experience.


Save Settings