Evidence that testicular sperm in infertile men has improved DNA integrity in comparison to ejaculated sperm
BAUS ePoster online library. Vyas L. 06/24/19; 259535; P4-6
Ms. Lona Vyas
Ms. Lona Vyas
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There is increasing evidence that the integrity of sperm DNA may be associated with recurrent miscarriage. The objective of this study was to determine the DNA quality of testicular versus ejaculated sperm.

Patients and Methods:
63 men with persistently raised DNA fragmentation from couples who had failed cycles of intracytoplasmic sperm injection (ICSI) with ejaculated sperm underwent testicular sperm extraction compared with 76 fertile donors. Sperm DNA fragmentation was measured by Comet assay. Average Comet scores (ACS), Low Comet scores (LCS) and High Comet scores (HCS) were determined. Comparisons were made for each of these parameters from ejaculated and testicular sperm from infertile men with fertile donors.


For total DNA damage ejaculate ACS (%) from infertile men was 40.3+/- 1.2 versus 17.9+/-1.3 in testicular sperm and 14.8+/- 0.6 from ejaculates from fertile donors (P<0.001).

Ejaculate LCS (%) from infertile men was 33.6+/- 2.4 versus 77.3+/-2.4 from testicular sperm and 90.2+/- 1.0 from ejaculates from fertile donors (P<0.001).

Ejaculate HCS (%) from infertile men was 29.5+/- 2.7 v 8.8+/-1.8 from testicular sperm and 1.9+/-0.4 from ejaculates of fertile donors (P<0.001).

Comparison of infertile ejaculate with fertile ejaculate was significant for all 3 groups of scores (P<0.001).

The total sperm DNA damage in testicular sperm from infertile men is not significantly different from ejaculated sperm from fertile donors (P=0.08).

This data provides compelling evidence that testicular sperm from infertile men have the same high DNA quality as ejaculated sperm from fertile donors.

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